Molecular Basis of Insulin-stimulated GLUT4 Vesicle Trafficking

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Divergent signalling mechanisms involved in insulin-stimulated GLUT4 vesicle trafficking to the plasma membrane.

4 Downes, C. P. (ed.) ( I 998) Special Lipid Signalling Issue Biochim. Biophys. Acta 1436, 1-264 5 De Camilli, P.. Emr, S. D., McPhenon, P. S. and Novick, P. ( 1996) Science 27 I, 1533I539 6 Lemmon. M. A., Falasca, M., Ferguson. K. M. and Schlessinger, J. ( 1997) Trends Cell Biol. 7, 237-242 7 Bottomley. M. 1.. Salim. K. and Panayatou, G. ( I 998) Biochim. Biophys. Acta 1436, 165I 83 8 Gaullier...

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Role for the microtubule cytoskeleton in GLUT4 vesicle trafficking and in the regulation of insulin-stimulated glucose uptake.

Insulin stimulates glucose uptake into adipocytes by promoting the translocation of the glucose transporter isoform 4 (GLUT4) from intracellular vesicles to the plasma membrane. In 3T3-L1 adipocytes GLUT4 resides both in an endosomal pool, together with transferrin receptors, and in a unique pool termed 'GLUT4 storage vesicles' (GSVs), which excludes endosomal proteins. The trafficking of GLUT4...

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Insulin-Regulated Trafficking of GLUT4 Requires Ubiquitination

A major consequence of insulin binding its receptor on fat and muscle cells is translocation of the facilitative glucose transporter GLUT4 from an intracellular store to the cell surface where it serves to clear glucose from the bloodstream. Sorting of GLUT4 into its insulin-sensitive store requires the GGA [Golgi-localized, γ-ear-containing, ADP ribosylation factor (ARF)-binding] adaptor prote...

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AS160 Phosphotyrosine-binding Domain Constructs Inhibit Insulin-stimulated GLUT4 Vesicle Fusion with the Plasma Membrane*

AS160 (TBC1D4) is a known Akt substrate that is phosphorylated downstream of insulin action and that leads to regulated traffic of GLUT4. As GLUT4 vesicle fusion with the plasma membrane is a highly regulated step in GLUT4 traffic, we investigated whether AS160 and 14-3-3 interactions are involved in this process. Fusion was inhibited by a human truncated AS160 variant that encompasses the firs...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1999

ISSN: 0021-9258

DOI: 10.1074/jbc.274.5.2593